Autophagy activation and cytotoxicity by gefitinib and chloroquine in PC-9/wt and PC-9/gefB4 cells.

<p>(A) PC-9/wt and PC-9/gefB4 were treated with gefitinib (100 nM) and chloroquine (CQ, 5, 10 μM) for 24 h. Total protein of treated cells was harvested. LC3-II levels were analyzed with Western blot assay. Each lane contained 30 μg protein for all experiments. Results were repeated in independent experiments. (B) PC-9/wt and (C) PC-9/gefB4 cells were cultured with gefitinib (100 nM) and CQ (5, 10 μM) for 96 h. (D) PC-9/wt cells were cultured with gefitinib (3 nM) and CQ (5, 10 μM) for 96 h. Cell viability was determined using SRB assay. Values are the mean ± S.E.M. (n = 3). *<i>P</i> < 0.05 statistically significant in the gefitinib or CQ-treated groups compared with the controls; # P< 0.05 statistically significant in gefitinib plus CQ-treated groups compared with gefitinib only groups.