Loss of function of <i>piga-1</i> shows similar phenotypes to <i>pigv-1(qm34)</i> mutant embryos.

<p>(A) <i>plag-2</i> promoter drives <i>piga-1</i> expression ubiquitously in various tissues: epidermis (top panel), intestine (middle panel) and excretory canal (bottom panel). (B) Loss of PIGA-1 expression results in elongations defects. Embryos were dissected from gravid adults expressing <i>plag-2</i>::<i>piga-1</i>::<i>gfp</i> and examined for their expression and phenotypes during embryogenesis. Those retaining PIGA-1::GFP expression elongate and hatch (top panel), whereas ~50% of embryos losing PIGA-1::GFP expression arrest during elongation with or without rupture (middle and lower panels).