eRF1 over-expression influences frameshifting.

<p>A. eRF1 transcript levels measured using quantitative real-time PCR. Results show non-transfected HEK293T cells (‘none’), and those transfected with pcDNA-eRF1 vector or pcDNA3.1(+) with no insert (empty vector). The mean ± SD for six replicates is shown. B. Immunoblot of eRF1 expression in non-transfected HEK293T cells (‘none’), or cells transfected with pcDNA3.1(+) with no insert (empty vector) or pcDNA-eRF1 (eRF1). The ratios were calculated after normalisation to β-actin and comparison with the non-transfected control (1.0). Raw data are available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122176#pone.0122176.s002" target="_blank">S2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122176#pone.0122176.s003" target="_blank">S3 Figs.</a> C. Effect of over-expression of eRF1 on readthrough at UGA test contexts. The mean ± SEM for four replicates (UGAAAG) or 12 replicates from three individual experiments (UGACUG) is shown. Cells were transfected with either empty vector (black) or pcDNA-eRF1 (grey). Values are shown above the bars for the strong stop signal UGAAAG. D. Effect of over-expression of eRF1 on +1 frameshift efficiency at the human antizyme frameshift element. The mean ± SEM for six replicates is shown. E. Effect of over-expression of eRF1 on HIV-1 −1 frameshift efficiency with GGG intercodon and when it is substituted with a stop codon (UGA or UAG). The mean ± SEM for six replicates is shown. pcDNA (black), pcDNA-eRF1 (grey). *<i>P</i> = < 0.05, **<i>P</i> = < 0.01, ***<i>P</i> = < 0.001, n.s., not significant.