Extracellular matrix-induced myelin sheet formation by oligodendrocytes requires Scribble.

<p>A,B; To assess differentiation of OPCs derived from neonatal rat pups into oligodendrocytes, cells were immunolabelled with antibodies against O4 (red) and myelin basic protein (MBP, green). C; Scribble knockdown did not change the proportion of O4-positive cells that express MBP on either poly-D-lysine (PDL, grey bars) or poly-D-lysine-Matrigel (PDL-MG, black bars) substrates. D; Culturing oligodendrocytes on PDL-MG increased the proportion of cells exuding myelin-like membrane sheets (arrows in A). While Scribble knockdown in oligodendrocytes cultured on PDL did not affect the proportion of cells with membrane sheets, the percentage of cells with membrane sheets (arrows in B) was significantly decreased on PDL-MG. Percent of O4-positive oligodendrocytes with myelin membrane sheets: non-targeting PDL = 13.6% ± 0.6%, non-targeting PDL-MG = 23.5% ± 0.8%, Scribble PDL-MG = 13.3% ± 0.9%. Numerical results are presented as mean ± standard error of the mean (SEM). ANOVA with Tukey's multiple comparison test was used, <i>n</i> = 6 coverslips per condition, with four fields analysed per coverslip. *** <i>p</i> < 0.005. Scale bar = 50 μm.