Wnt3a-mediated inhibition of HSPC expansion is due to activation of the canonical Wnt3a pathway.

<p>(A) Expansion of CD34⁺ cells after 7 and 14 days of culture in SFT, SFT+Wnt3a, SFT+Fr8CRD and SFT+Wnt3a+Fr8CRD (n = 2). (B) CD34⁺ cell frequency within the TNC population during these cultures (n = 2). (C) CD34⁺ cell expansion in SFT and SFT+CHIR99021 after 7 and 14 days of culture (n = 2).