OCH administration increased cell infiltrates and activation of T cells in mice.

<p>(A) C57BL/6 mice were intravenously injected with α-GalCer, OCH, or PBS. Liver total mononuclear cells (MNC) were counted 3 days after α-GalCer, OCH, or PBS injection. n = 10–13 mice per group. ***, p<0.001. (B-E) Wild type mice were immunized with 2-OA-BSA and α-GalCer (group name: 2-OA/a-GC), OCH (group name:2-OA/OCH) or PBS (group name: 2-OA/PBS) at weeks 0, 2, 4, 6 and 8 and sacrificed at week 12. (B) Liver total mononuclear cells (MNC) were measured. (C) The numbers of T (CD3⁺ NK1.1^-), NKT (CD3⁺NK1.1⁺), NK (CD3^-NK1.1⁺) and B (CD19⁺) cells were measured. (D) The numbers of CD4⁺ and CD8⁺ T cells were detected. (E) The expression of CD69 and CD44 in CD4⁺ and CD8⁺ T cells was measured by flowcytometry. n = 9–10 mice per group. *, p<0.05; **, p<0.01; ***, p<0.001.