The different sensitivity to nutrient deprivation (ND) caused by YAP is attenuated after autophagy inhibition.

<p><b>(A)</b> MCF7 cells were cultured under the indicated conditions for 36 h without chloroquine (CQ) or for 18 h with CQ, stained by Annexin V-FITC/PI, and analyzed with flow cytometry. Representative results are shown. <b>(B)</b> Quantitative analysis of percentages of apoptotic cells according to results of (A) are expressed as the percentage of Annexin V-positive cells out of the total number of cells in the gate. Data are shown as the means±SEM. *<i>P</i><0.05, n = 3. <b>(C)</b> After 38 h (without CQ) or 18 h (with CQ) of indicated treatments, MCF7-shCtrl, shYAP and YAP Res cell viability was determined by MTT assay. *<i>P</i><0.05, n = 3. <b>(D)</b> After 38 h (without CQ/NH₄Cl) or 18 h (with CQ/NH₄Cl) of indicated treatments, MCF7-shCtrl, shYAP and YAP Res cells were stained by Annexin V-FITC/PI and analyzed with flow cytometry. <b>(E)</b> Quantitative analysis of percentages of apoptotic cells according to the results of (D) was assayed as previously. Data are shown as the means±SEM. *<i>P</i><0.05, n = 3. <b>(F)</b> MCF7-shCtrl and shYAP cells were transfected with control (Ctrl) and LC3 siRNA for 48 h and treated with ND for 36 h. Cells were then stained by Annexin V-FITC/PI and analyzed with flow cytometry. Quantitative analysis of percentages of apoptotic cells is derived from triplicates. *<i>P</i><0.05, n = 3. <b>(G)</b> MCF7-shCtrl and shYAP cells were transfected with control (Ctrl) and LC3 siRNA for 48 h and cultured in normal medium or EBSS (ND) for 36 h. Cell viability was determined by MTT assay. *<i>P</i><0.05, n = 3. <b>(H)</b> MCF7-shCtrl and shYAP cells were transfected with the indicated siRNA for 48 h and cultured in normal medium or EBSS (ND) for 36 h. Representative blots are shown. The experiment was repeated twice.