Ion homeostasis gene expression for the control animals that received no treatment three hours and six hours after transtympanic inoculation with <i>H flu</i>.

<p>This reflects fold change in gene expression seven and ten hours after inoculation compared to animals receiving no bacteria. Although there were some genes that were expressed at higher than normal levels (claudin 4, gap junction a1, aquaporin 1), the majority of homeostasis genes in the middle ear were suppressed in expression. Many other genes were reduced to 10–30% of normal expression (Clcnka, claudin 14, aquaporins 2 and 3, most postassiumchannels, and ENac channels Scnn1b and Scnn1g), but these did not reach statistical significance due to variability. In general, the expression levels seen in the three hour controls were similar to those in the six hour controls.