Requirement of IRF3-induced host genes for <i>T</i>. <i>gondii</i> replication.

<p><b>A,</b> Infection and immunoblotting was performed with HDAC6 KO and wild type MEF cells. <b>B</b>, MEF cells (wild type or IFNAR KO) were infected with <i>T</i>. <i>gondii</i> and the indicated proteins were measured by immunoblotting. <b>C</b>, <i>T</i>. <i>gondii</i> growth measured in IRF3 KO MEF cells; where indicated, recombinant murine IFN-β (2,000 U/ml, R&D Systems) was added, and parasite was added 6 h later. Immunoblotting was performed as above. Where shown, parasite growth was also quantified by qPCR of genomic DNA, and ISG56 (Ifit1) mRNA was quantified by qRT-PCR as described before [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004779#ppat.1004779.ref066" target="_blank">66</a>].