Deletion analysis of the <i>UME6</i> promoter identifies condition-specific response elements.

<p>Strains bearing the indicated <i>UME6</i> promoter-<i>lacZ</i> reporter constructs were grown under non-inducing conditions (YEPD at 30°C for the serum and temperature induction experiment and Lee’s pH 4.5 medium for the neutral pH induction experiment) and the indicated filament-inducing conditions. Cells were harvested (at 30 minutes for the serum and temperature induction experiment and at 1 hour for the neutral pH induction experiment) for total RNA isolation and cDNA synthesis. <i>lacZ</i> expression values were determined by qRT-PCR and normalized to <i>ACT1</i> levels. Fold up-regulation of <i>lacZ</i> was determined by dividing normalized <i>lacZ</i> values from cells induced to form filaments under each filament-inducing condition by normalized <i>lacZ</i> values from cells grown under non-filament inducing conditions. Please note that the fold <i>lacZ</i> up-regulation value for the—6.0 kb <i>UME6</i> promoter construct in 37°C + serum has been reported previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122775#pone.0122775.ref038" target="_blank">38</a>].