Snx10 is expressed in the stomach and is required for gastric acid production and calcium homeostasis.

<p>A) Representative stomachs from 3-week old Snx10 KD mice (n = 6) and WT littermates (n = 6). Snx10 KD mice have abnormal stomachs. B) mRNA expression of Snx10 (left panel) is mainly observed in stomach and bone. Within the stomach (right panel) expression of Snx10 mRNA is mainly observed in the body/corpus of the stomach ("Body"), where levels are comparable to the femur; expression is lower in the antrum and the non-glandular forestomach. C) Immunofluorescence images from stomach sections stained for pepsinogen (a zymogenic cell marker), Snx10 and GSII (a mucous neck cell marker) shows Snx10-specific staining (green) in zymogenic cells. D) Sections of the body of the stomach stained for the parietal cell marker VEGF-B (red), show that Snx10-deficient parietal cells are smaller, have abnormal nuclear morphology, increased nuclear to cytoplasmic ratio, and an abnormal cellular distribution of VEGF-B. E) Gastric pH is significantly elevated in Snx10 KD mice indicating that Snx10 is required for gastric acid production (* P<0.05). F) Serum calcium is reduced in Snx10 KD mice, indicating that Snx10 is required for calcium homeostasis (*P<0.05). G) Serum parathyroid hormone (PTH) is significantly elevated in Snx10 KD (* P = 0.001). H) 1,25-dihydroxyvitamin D levels are reduced in Snx10 KD mice (* P = 0.01).