Effect of <i>Mstn</i> knockdown on the signals in the sheep myoblasts at the mRNA level.

<p>Sheep myoblasts infected by the <i>Myostatin</i> shRNAs (si-<i>mstn</i>) or control (ctrl) vectors were cultured in either GM or DM for 3 days. Cell lysates were used for gene expression by qPCR. Gene expression was quantified relative to <i>ACTB</i> expression using the 2-△△Ct method. The data are shown as the mean + SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001.