Proximity ligation assay for cN-II-Ipaf interaction in MCF7 cells.

<p>Cells were transiently co-transfected with the empty vector pcDNA3 and pEGFP-C1 (ratio 10:1, upper panel) or with pEZ-M02 Ipaf and pEGFP-C1 (ratio 10:1, lower panel) and subsequently analyzed for protein-protein interaction by PLA in GFP-positive cells. Red dots in PLA signal correspond to the detection of cN-II interacting with Ipaf; DAPI corresponds to the nuclear staining of the cells; BF is the image of the cells in bright field; GFP shows GFP-positive cells; Merge corresponds to the merging of PLA, DAPI and GFP images.