Time course and concentration dependence of nicotine activation of EGR-1 in SH-SY5Y cells.

<p>a, b) Nicotine activates EGR-1 in the nanomolar to micromole range when SH-SY5Y cells were treated with nicotine for 30 min; the relative level of EGR1 was quantified and presented in panel b. β-actin serves as control. c-e) The SH-SY5Y cells were with 10^-6M nicotine by the indicated time, the relative level of EGR1 was quantified and presented in panel d and f respectively. β-actin serves as control. g) The mRNA level of EGR1 in SH-SY5Y cells after nicotine treatment was measured by RT-PCR. i) Immunofluorescence was shown when SH-SY5Y cells were treated by nicotine compared to control.