CCR2 inhibitor ameliorates insulin resistance in experimental mice.

<p>(A, B and C) Western blots of insulin signaling (pIRS-1 Ser³⁰⁷, IRS-1, pAkt and Akt) in liver from CCR2 inhibitor-treated (<i>n</i> = 9) and control (<i>n</i> = 8) <i>db/db</i> mice. (D) Western blots of mTOR in liver from CCR2 inhibitor-treated (<i>n</i> = 9) and control (<i>n</i> = 8) <i>db/db</i> mice. (E) GLUT4 mRNA in the livers of treated <i>db/db</i> mice. mRNA levels were evaluated by real-time reverse transcriptase-PCR. Bands intensities were measured with an Image J Analyzer. Results shown are means ± SEM (n = 8–9 mice per group). *P < 0.05 compared with <i>db/m</i> mice. #P < 0.05 compared with control <i>db/db</i> mice.