Global identification of mRNA transcriptional start sites (TSSs).

<p>(A) Visualization of RNA-seq (+/- TAP) based cDNA sequencing reads mapped to the YPIII <i>rovA</i>, <i>hfq</i>, <i>katY</i> and <i>crp</i> gene locus using the Artemis genome browser (Release 15.0.0). (B) The 5’-UTR repertoire. The distribution and frequency of the length of 5’-UTRs is given for all mRNAs of YPIII which start upstream of the annotated translational start site. More than 40% of all 5’-UTRs are 20–60 nt in length. (Inset) Sequence conservation at the TSSs. Sequence logo computed from 1151 unaligned TSS regions (TSS is located at position +1) showing nucleotide conservation around the TSSs. The initial nucleotide of transcripts (position +1) is dominated by purines while position -1 is dominated by pyrimidines. (C) Detected conserved sequence motifs in the -35 and -10 promotor region.