Vaccine-induced immunity requires IL-25.

<p><b>A</b>. Anti-HES pre-challenge IgG1 titres in control and vaccinated C57BL/6 and IL-17RA-deficient mice. <b>B, C</b>. Day 28 worm counts and fecal egg burdens (d 14, 21 and 28) in control and vaccinated C57BL/6 and IL-17RA-deficient mice. <b>D</b>. Intracellular IL-4 production by naïve and d 7 post-challenge CD45⁺CD4⁺ lamina propria cells from C57BL/6 and IL-17RA^–/– mice. Pooled from two experiments. <b>E</b>. Proportion of lamina propria CD45⁺CD11b⁺Ly6G⁺ in naïve, d 3 and d 7 challenged C57BL/6 and IL-17RA^–/– mice. <b>F</b>. Adult worm burdens (d 16) in control and vaccinated C57BL/6 mice treated with anti-Ly6G (clone 1A8; left) or anti-Gr-1 (clone RB6-3C5; right) as detailed in materials and methods. <b>G</b>. Adult worm burdens (d 28) in primary infected BALB/c mice treated with anti-Ly6G, anti-Gr-1, or rat IgG control, as in (F). <b>H</b>. Day 28 worm counts in control and vaccinated C57BL/6 and IL-25-deficient mice. Significance in (A-E, G-H) determined by unpaired <i>t</i>-test or Mann-Whitney test, significance in (F) determined by ANOVA <i>Vs</i> PBS control. Data from A-G pooled from 2 independent experiments. See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004676#ppat.1004676.s004" target="_blank">S4 Fig</a>.