Confocal projection images of the tympanic membrane and mucosa of the middle ear in control and LPS-treated mice.

<p>Confocal maximum projection images of the tympanic membrane and mucosa of the middle ear in control (A, B and C) and LPS-treated mice (D, E and F). (A) is a low magnification image showing the normal tympanic membrane (TM) and mucosa in the middle ear (mucosa/ME) of a control animal. (<b>B</b>) and (<b>C</b>) are high magnification images of the TM and mucosa/ME in a control animal. (<b>D</b>) is a low magnification image showing thickening of the TM and swelling of the mucosa/ME typically observed in LPS-treated animals. (<b>E</b>) and (<b>F</b>) are high magnification images showing a large number of infiltrated cells expressing F4/80 (red, arrow) and positive for GS-IB4 (green, arrow) in the region of the TM and mucosa/SEM in LPS-treated animals. TM = tympanic membrane; ME = middle ear.