Immunophenotyping of memory and differentiation status of the specific CD8⁺ T cells in patients with Chagas disease.

<p>(A) TcCA-2_442-451 peptide. (B) TcCA-2_607-615 peptide. PBMCs from 6 asymptomatic form patients (IND) and 11 patients with the cardiac form (CCC) were stained for CD45RA, CD27 and CCR7 and analyzed by flow cytometry. According to the combination of antibodies used the CD8 peptide-specific cells were divided in: T_NAIVE (CD8⁺CD45RA⁺CD27⁺CCR7⁺), T_EMRA (CD8⁺CD45RA⁺CD27^-CCR7^-), T_CM (CD8⁺CD45RA^-CD27⁺CCR7⁺), T_EM (CD8⁺CD45RA^-CD27^-CCR7^-), T_ED (CD8⁺CD45RA^-CD127⁺), T_TD (CD8⁺CD45RA⁺CD127^-). Median values are represented by horizontal lines. Error bars represent standard deviation intervals. Statistical analyses were carried out using Mann-Whitney U test (#) and Kruskal—Wallis test with Dunn correction (*). Statistically significant differences are indicated ^(#) or ⁽*⁾ p≤0.05, ^{(# #)} or ⁽**⁾ p≤0.01, ^{(# # #)} or ⁽***⁾ p≤ 0.001.