Body weight and tissue weights in MC4R-KO mice treated with EPA for 24 weeks.

<p>(A) Experimental protocol of preventive EPA treatment. Growth curve (B) and weights of the subcutaneous, epididymal and mesenteric white adipose tissues (C) and liver (D) of male MC4R-KO (MC4R) and wildtype (WT) mice. WT-SD, WT mice fed standard diet (SD); MC4R-control, MC4R-KO mice fed Western diet (WD) supplemented with 5% (wt/wt) palmitate; MC4R-EPA Pre, MC4R-KO mice fed WD supplemented with 5% (wt/wt) EPA for 24 weeks. Open circle, WT-SD; Open triangle, MC4R-control; closed square, MC4R-EPA Pre. (E) Liver triglyceride (TG) content at 24 weeks. (F) Insulin tolerance test (ITT) at 12-week WD feeding. Open triangle, MC4R-control; closed square, MC4R-EPA Pre. ** <i>P</i> < 0.01; n.s., not significant. WT-SD, <i>n</i> = 8; MC4R-control, <i>n</i> = 7; MC4R-EPA Pre, <i>n</i> = 10.