Protein content and mRNA levels of VAChT. A and B.

<p>VAChT mRNA expression (A) measured by real-time PCR (6–8 mice per group) and protein content (B) quantified by Western Blot (3 mice per group) in spinal cord from wild-type and VAChT-KD^HOM (mutant). <b>C and D</b>. VAChT mRNA expression (C) measured by real-time PCR (6–8 per group) and protein content (D) quantified by Western blot (6–8 mice per group) in lung from wild-type and VAChT-KD^HOM (mutant). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a housekeeping for gene expression analysis (A and C) and β-actin was used as protein loading control for Western Blot (B and D). Both were presented as a percentage of WT. The gels (B and D) is representative of results that were obtained in an experiment that was repeated two times. *p<0.05 vs wild-type mice. <b>E and F:</b> Body weight in g (E) in wild-type and mutant mice and the time in wire hang test performed before all measurements in seconds (F). E and F represent data of 8–14 animals per group. Data area expressed as means ± SEM. *P<0.001 vs. wild-type mice.