Kinetic analysis of genomic RNA fate.

<p><b>A</b>. Huh-7.5 cells were infected with HCV at MOI = 1.5. RNA was collected at the indicated time points post-infection and quantified with real-time RT-PCR. Error bar, standard deviation. <b>B</b>. Intra- and extra-cellular viral supernatants from infections in panel A were collected at the indicated time points and titered by limiting dilution assay. Shown are the averages of two sets of titer data. Error bar, standard deviation. <b>C</b>. Total (+) puncta or <b>D</b>. Percent of (+) strand puncta colocalizing with translation (puromycylated ribosomes), replication (NS5A + NS3), assembly (core) and virion (E2) markers over the indicated time course (Figs. <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004758#ppat.1004758.g003" target="_blank">3</a>–<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004758#ppat.1004758.g006" target="_blank">6</a>) were plotted using the smooth graph function in Microsoft Excel. <b>E</b>. Percent of (−) strand puncta colocalizing with active replication compartments (NS5A), active replication ((+) strands) and active replication (NS3) over the indicated time course plotted using the smooth graph function in Microsoft Excel.