Delivery of EGFP into the macrophage cell line J774A.1 through rSmeg or rBCG carrying the pMyong2-EGFP^h shuttle vector.

<p>GFP expression in rSmeg harboring the pMyong2-EGFP^h vector was observed using (A and B) fluorescence and (C and D) confocal microscopy. A FACS analysis of the GFP expression of rSmeg harboring (E, F, and G) pMyong2-TOPO and (H, I, and J) pMyong2-EGFP^h at 10, 50, and 100 M. O. I., respectively. In addition, GFP expression in <i>M</i>. <i>bovis</i> BCG harboring (K, L and M) pMyong2-TOPO or (N, O, and P) pMyong2-EGFP^e was analyzed at 10, 50, and 100 M.O.I., respectively.