EGFP protein expression by rSmeg or rBCG harboring the pMyong2-EGFP^h vector.

<p><i>M</i>. <i>smegmatis</i> transformed with the pMyong2-EGFP^h vector was observed using (A and B) fluorescence and (C and D) confocal microscopy. <i>M</i>. <i>bovis</i> BCG carrying the pMyong2-EGFP^h vector was also observed using (G and H) fluorescence and (I and J) confocal microscopy. The GFP expression levels in <i>M</i>. <i>smegmatis</i> and <i>M</i>. <i>bovis</i> BCG, transformed with (E and K) pMyong2-TOPO or (F and L) pMyong2-EGFP^h, were detected using flow cytometry. (M) GFP expression levels in each generation of rSmeg transformed with pMyong2-EGFP^h were analyzed using flow cytometry. Abbreviations: G1, 1^st generation; G2, 2^nd generation; G3, 3^rd generation; G4, 4^th generation; G5, 5^th generation. Each generation time was 3 days.