Molecular characterization of the plasmid from <i>M</i>. <i>yongonense</i> DSM 45126^T.

<p>(A) The read-length coverage of pMyong2 was compared with the chromosome and circular pMyong1 plasmid DNA. (B) Southern blot analysis was used to assess the presence and validity of the linear plasmid, pMyong2. The left panel shows the results of gel electrophoresis, and the right panel shows the results of Southern blot analysis. The 1-kb DNA ladder (M), <i>M</i>. <i>intracellulare</i> genomic DNA (lane 1), <i>M</i>. <i>yongonense</i> genomic DNA (lane 2), and <i>Xho</i>I-digested <i>M</i>. <i>yongonense</i> genomic DNA (lane 3) are shown. (C) Comparison of the replication origin sequence in pMyong2 with those of other mycobacteria plasmids. The <i>rep</i> gene is indicated with an open arrow. The arrowheads indicate the 18-bp repeat sequences. The AT-rich segment of the replication origin region is indicated as ‘A+T’ in red. A comparison of the conserved sequences in pMyong2 with those of other mycobacterial plasmids, including pCLP (GenBank Accession No., AF312688), pJAZ38 (GenBank Accession No., MFU85216), pMF1 (GenBank Accession No., AJ238973), pVT2 (GenBank Accession No., AY0565023), and pMSC262 (GenBank Accession No., D14416), is shown. Nucleotides identical to the pMyong2 sequence are indicated with black backgrounds.