Application of the microcultivation growth assay to the analysis of salt resistance in several yeast species and inter-experimental reproducibility of this assay.

<p>A) Growth was measured in a microplate reader using liquid cultures supplemented with increasing NaCl concentrations (w/v) in 384-well plates for <i>S</i>. <i>cerevisiae</i> (FY4 strain), <i>P</i>. <i>farinosa</i> and <i>S</i>. <i>kluyveri</i>. For the sake of clarity, the curves shown were not corrected for background absorbance or non-linearity of OD measurements at high cell density. B) Growth was measured using this same assay for various <i>S</i>. <i>cerevisiae</i> strains (FY and W303 strains in both haploid and diploid states) and other yeast species (<i>K</i>. <i>lactis</i>, <i>P</i>. <i>farinosa</i>, <i>P</i>. <i>sorbitophila</i>, <i>S</i>. <i>kluyveri</i>, <i>Z</i>. <i>rouxii</i>) cultivated in the presence of different NaCl concentrations in two identical, but independent experiments. Growth parameters were determined using the GATHODE software. The values shown are means of three biological replicates.