A) Analysis of CagA expression and CagA tyrosine phosphorylation in the <i>ccrp59</i> mutant.

<p>Bacterial lysates from <i>H</i>. <i>pylori</i> wild type KE and <i>ccrp59</i> mutant KE-59PCAT were prepared respectively. Each sample that consisted of equivalent amounts of protein was subjected to immunoblotting assay using antiserum against CagA. AGS cells were not infected or infected with these strains at an MOI of 100 for 4h and subjected to immunoblotting analysis using specific antibody against phosphorylated CagA (Cag-P). B) Control experiment showing that CagA of the <i>ccrp59</i> mutant can be phosphorylated in vitro by mixing <i>H</i>. <i>pylori</i> lysates with AGS cell lysates.