PQQ suppresses RACK1 expression in activated HSCs by inhibiting NF-κB pathway.

<p>(A) Liver sections were stained with RACK1 (red) and α-SMA (green) antibodies. Scale bar, 100 μm. (B) Freshly isolated HSCs from different groups of mice were applied to western-blot and qPCR analysis to detect RACK1 expression. (C, D) Primary mouse HSCs were treated with 10 ng/ml TGF-β1 for 24 h, along with or without increasing doses of PQQ or NAC. Total cell lysates or nuclear extracts were subjected to western blot analysis (C) and ChIP assay (D). N, normal group. M, TAA model. L, low-dose PQQ. H, high-dose PQQ. S, silymarin. *, p<0.05, **, p<0.01.