Immunostaining of parietal cell H⁺/K⁺-ATPase in fundic mucosa.

<p><b>(A)</b> Overview of a representative immunostaining of parietal cell H+/K⁺-ATPase along the formalin-fixed gastric pouch anastomosed to jejunum in the alimentary limb after RYGB. Comparative immunostaining of parietal cell H+/K⁺-ATPase in fundic mucosa from sham <b>(B)</b>, residual stomach after VSG <b>(C)</b>, and GP after RYGB <b>(D)</b>. Note the enlarge parietal cells <i>(blue stars)</i> with dense H⁺/K⁺ATPase immunoreactivity as compared to sham. <b>(E)</b> Percent of H⁺/K⁺-ATPase positive cells per fundic mucosa area (μm²). Values are shown as mean ± SEM n = 5 for sham and n = 6 for VSG and RYGB. **P<0.01 and ***P<0.001 <i>vs</i>. sham.