Levels of ghrelin and gastrin mRNA in remodeled stomach after VSG and RYGB.

<p><b>(A)</b><i>Insert</i>: <i>scheme with location of fundus and antrum in normal stomach</i>, <i>residual stomach after VSG and gastric pouch (GP) or excluded stomach after RYGB</i>. <b>(A)</b> Quantification of <i>ghrelin</i> mRNA in mucosa scrapings of residual fundic mucosa after VSG, fundic mucosa in gastric pouch (GP) after RYGB and antral mucosa of the excluded stomach after RYGB in HFD-obese operated rats in comparison to fundic and antral mucosa from sham-operated ND or HFD rats. <b>(B)</b> Quantification of <i>gastrin</i> mRNA in mucosa scrapping from antrum of sham-operated ND and HFD rats, antrum of residual stomach after VSG, and antrum of excluded stomach after RYGB. L19 was used as reference. n = 5 rats per group. <b>(C)</b> Representative photomicrographs of immunfluorescence of gastrin-positive cells in antral mucosa of sham- and VSG-operated HFD rats. <b>(D)</b> Comparative number of gastrin positive cells per antral mucosa area (μm²) showing significant decrease in G cell number. Values are means ± SEM for n = 4 in each group.