Fibroblast proliferation and collagen synthesis assays in different culture conditions.

<p>A and B. The growth curves and collagen synthesis of fibroblast cultured in un-conditioned or conditioned (stimulated or un-stimulated) medium; *P<0.05, **P<0.01, ***P<0.001 compared with un-conditioned group. C and D. The growth curves and collagen synthesis of fibroblast cultured in un-stimulated or stimulated (add anti-MIF antibody or not) medium; *P<0.05, **P<0.01, ***P<0.001 compared with stimulated group without anti-MIF antibody. E and F. The growth curves and collagen synthesis of fibroblast cultured in medium with or without MIF supplement, mean±SD, n = 3.