Quantitative proteomic analysis of macrophage nuclei after infection with <i>L</i>. <i>major WT</i> or <i>L</i>. <i>major</i> GP63^-/-.

<p>(A) Comparison of nucleoplasmic proteins: <i>L</i>. <i>major</i> WT samples vs Nil samples. All proteins identified in Nil samples were represented according to their emPAI value (smallest to highest) as green diamonds. All proteins identified in <i>L</i>. <i>major</i> WT were represented as yellow triangles, according to the protein order of Nil samples. This allows visualizing which proteins are unique, smaller or higher in abundance in the Maj WT samples compared to the Nil ones. (B) Comparison of nucleoplasmic proteins: <i>L</i>. <i>major</i> GP63^-/- samples vs Nil samples. Analysis was carried out as in A with <i>L</i>. <i>major</i> GP63^-/- proteins identified represented as pink triangles. This allows visualizing which proteins are unique, smaller or higher in abundance in the Maj GP63^-/- samples compared to the Nil ones. (C) Venn diagram of proteins identified in Nil, <i>L</i>. <i>major</i> WT and <i>L</i>. <i>major</i> GP63^-/- samples. (D) Analysis of the changes in emPAI values of <i>L</i>. <i>major</i> WT and <i>L</i>. <i>major</i> GP63^-/- samples. Displayed is the number of proteins (in %) of the <i>L</i>. <i>major</i> WT samples (yellow) and <i>L</i>. <i>major</i> GP63^-/- samples (pink) according to the fold-change of their emPAI value in comparison to Nil samples. Blue lines correspond to -1.5x and +1.5x fold-change, which were considered as significant values. (E) Comparison of nucleoplasmic proteins: <i>L</i>. <i>major</i> WT samples vs <i>L</i>. <i>major</i> GP63^-/- samples. All proteins identified in <i>L</i>. <i>major</i> GP63^-/- (and Nil) samples were represented according to their emPAI value (smallest to highest) as pink diamonds. All proteins identified in <i>L</i>. <i>major</i> WT samples were represented as yellow triangles, according to the <i>L</i>. <i>major</i> GP63^-/- sample protein order. This allows visualizing which proteins are unique, smaller or higher in abundance in the Maj WT samples compared to the Maj GP63^-/- ones.