CFCA measurements of IgG subgroups with high ligand affinity.

<p>CFCA raw SPR data and fits of 4G8a association using a 833-fold stock dilution in a flow cell with immobilized SNA lectin (A) and a 3333-fold stock dilution in a flow cell with immobilized OAβ (B). The raw data and fits at flow rates 5 μl/min and 100 μl/min are indicated along with the QC parameter and percent standard error SE of the CFCA fit. The concentration of the original 4G8a stock determined from UV measurements was 6500 nM.