On-Chip SPR measurement of IgG surface sialic acid.

<p>A representative SPR On-Chip experiment of a 66-fold dilution of 4G8a in a flow cell with immobilized OAβ. In Step 1, IgG (4G8a) is bound for 120 s and the association binding signal, RU_IgG, is measured. In Step 2, 3 μM SNA lectin is bound for 180 s to the flow cell containing IgG and this second association signal, RU_L+IgG, is measured. The background binding signal of 3 μM SNA, RU_L-IgG, is measured for the flow cell in the absence of IgG (grey curve/arrow).