Tks5 expression in breast tissue and breast cancer.

<p>A) 293 cells were transfected with empty vector, or vectors expressing Tks4 or Tks5. Cells were pelleted, fixed, embedded in paraffin and processed for immunohistochemistry with an anti-Tks5 antibody. B) Representative images of normal breast lobules and duct, and ductal carcinoma in situ, stained with anti-Tks5 antibodies. Red arrows indicate areas with intense Tks5 staining. Scale bar represents 100μm. C) Representative images of primary invasive breast cancer specimens, at low and high magnification, to illustrate the range of Tks5 expression observed. Scale bars represent 100μm. Quantification of 163 specimens is shown on the right. D) Kaplan-Meier survival curves for patients with high (red) and low (black) Tks5α mRNA levels. E) Kaplan-Meier survival curves for stage I/II patients with high (red) and low (black) Tks5α mRNA levels. F) Kaplan-Meier survival curves for stage III/IV patients with high (red) and low (black) Tks5α mRNA levels.