Investigation of antigen-specific regulatory function in antigen-immunized mice.

<p>Inhibition of CD4⁺CD25^- effector T cell proliferation by CD4⁺CD25⁺ regulatory T-cells isolated from the spleen of control (GST-Den–immunized) and construct-immunized mice when AHHC, RHHC and RPHC were used as antigens. (A) Quantitative analyses of proliferation of CD4⁺CD25^- effector T cells in the presence of Treg cell by flow cytometry is shown. (B) Proliferation of effector cells isolated from immunized mice alone is indicated in the leftmost bar of each group. Addition of Treg cells to T effector cells at different ratios was also shown. Data are given as mean of 3 analyses ± SEM.