Assessment of inflammation-associated cells in the lesions of <i>B6;129S-Ldlr</i>^{<i>tm1Her</i>}<i>Apob</i>^{<i>tm2Sgy</i>}<i>/J</i> mice mice fed a high-fat diet after immunization with constructs AHHC, RHHC, and RPHC.

<p>(A) Photomicrographs showing IHC staining of CD68 (green) and CD11c (red) markers (scale bar: 100 μm and 12.5μm for magnified ones). (B) Scatter plot showing anti-CD68-stained area in lesion versus total lesion area; Data are given as the mean of 6 mice. (C) Scatter plot showing anti-CD11c-stained area in lesion versus total lesion area; Data are given as the mean of 6 mice. (D) Co-localization of CD68⁺ and CD11c⁺ areas (derived from Fig 3B and 3C). (E) Photomicrographs showing IHC staining of CD4⁺ T-cells (green) and Foxp3⁺ Treg cells (red) (scale bar: 100 μm and 12.5μm for magnified ones). (F) Scatter plot showing anti-Foxp3-stained area versus anti-CD4⁺ stained area in lesion (N = 6). (G) Representative analysis of Foxp3 expression by CD4⁺ T cells in lymph nodes from construct-immunized mice fed on a high-fat diet as assessed using flow cytometry. (H) Percentage of Foxp3⁺ cells among CD4⁺ spleen cells as analyzed by flow cytometry. Data are expressed as mean of 3 analyses ± SEM. Differences between groups are shown.