Treatment with SMIFH2 ablates formation of the cortical granule free domain (CGFD) and decreases the cortical tropomyosin levels.

<p><b>A.</b> Treatment with SMIFH2 impairs the formation of the cortical granule-free domain (CGFD) in comparison to control oocytes (left). Oocytes treated with 500 μM of SMIFH2 failed to form the CGFD, which is stained with labeled lectin (green). Chromatin was visualized with Hoechst 33342 (blue). Bar: 20 μm. <b>B.</b> CGFD formation (%) in control oocytes (n = 13) and in oocytes treated with 500 μM of SMIFH2 (n = 12). <b>C.</b> Treatment with SMIFH2 affects the cortical tropomyosin levels. In control oocytes (left) and oocytes treated with 500 μM of SMIFH2, Tpm3-2 in cortical actin was immunostained (green) and DNA was stained with Hoechst 33342 (blue). Bar: 20 μm. <b>D.</b> Intensity of the cortical tropomyosin in control oocytes (n = 17) and in oocytes treated with 500 μM of SMIFH2 (n = 10). The box represents the interquartile range; the whiskers show 1.5x the interquartile range; line inside the box represents the median.