HPLC analysis shows MLT oxidation thereby preventing MPO heme destruction and generation of free iron.

<p>A) HPLC trace for MLT (elution time 3.98 min) dissolved in DMF (elution time 3.31 min) and phosphate buffer (elution time 2.48 min). B) Addition of MPO and Cl^- causes no significant change in MLT peak intensity and/or retention time. C) Addition of H₂O₂ (sequential addition of 20 μM, total 200 μM) results in a significant shift in MLT retention time elution time (3.71 min) as well as the appearance of a small peak around 3.57 min. D) Increasing levels of H₂O₂ (400 μM) resulted in the domination of the MLT metabolite eluted at 3.57 min showing the retention and absorbance properties of AFMK (elution time 3.57 min) as shown in panel (E).