Influence of ghrelin treatment (0.5 μg/g BW, twice a day, intraperitoneal (ip.)) on peritoneal cellular immune response after cecal ligation and puncture (CLP)-induced sepsis.

<p>Peritoneal lavage fluid from control mice (ghrelin: n = 5/timepoint, untreated: n = 5–11/timepoint) and high fat diet (hfd) mice (ghrelin: n = 5/timepoint, untreated: n = 4–10/timepoint) were collected before, 24h and 48h following CLP. Numbers of neutrophils of control (A) and (B) hfd mice, natural killer (NK) cell numbers of (C) control and (D) hfd mice and γδ T cell numbers of (E) control and (F) hfd mice were were enumerated by flow cytometric analysis (combined data from two independent experiments). All values are expressed as means ± SEM; * p < 0.05 of unpaired t-test for comparison of baseline levels between two groups (hfd and control) and of two-way ANOVA for unmatched samples followed by a <i>post hoc</i> Bonferroni test for analyses between more than two groups or within groups over time.