tDC generated by nucleosides present ability to induce regulatory profile on CD4⁺CD25^- cells.

<p>Bone marrow-derived cells (BMDC) treated with ADO or PBS and stimulated with LPS (50 ng/ml) were co-cultured with CD4⁺CD25^-under Treg polarizing conditions at the ratio of 1:10 (BMDC:T cells). Natural Treg (nTreg, CD4⁺CD25⁺) or Th0 (CD4⁺CD25^-) were used as positive and negative differentiation control. Representative histograms of CD39, CD73, CD103, and FoxP3 are shown in each box. Bars display the relative mean fluorescence intensity, and the results are expressed as the mean ± SEM obtained from one of three independent experiments made in triplicate (<i>n</i> = 3 per group).*, <i>p</i><0.05 relative to the BMDC/PBS group.