ADO+AMP exacerbates <i>Leishmania amazonensis</i> infection in vivo.

<p>C57BL/6 or BALB/c mice were infected with 10⁶<i>L</i>. <i>amazonensis</i> promastigotes in the presence of ADO+AMP (●) or PBS (Ο). The infection course was monitored weekly by measuring sizes of ear lesions with metric calipers. In <i>A</i> and <i>D</i>, lesion size was determined by the difference in thickness between the infected ear and the opposite uninfected ear on each mouse. Measurements are reported in millimeters (mm); <i>n</i> = 4 mice per group. Data are shown as the mean ± SEM and are representative of three different experiments. *, <i>p</i><0.05 relative to the PBS-treated group. Parasite burdens in ears (<i>B</i>) and draining lymph nodes (<i>E</i>) at 11 weeks post infection were determined by a limiting-dilution assay. Data are shown as the means ± SEM of three separate experiments; each experiment was performed with five mice per group <i>n</i> = 5. <i>C</i> and <i>F</i> are representative photographs of mouse ears at 11 weeks post infection. <i>In</i> G and H, BALB/c mice were infected with 10³ metacyclic promastigotes forms of <i>L</i>. <i>amazonensis</i> in the presence of ADO+AMP (●) or PBS (Ο). In I-J, as in D–E, but using infection with 10⁶ stationary-phase <i>L</i>. <i>major</i> promastigotes. Data are shown as the means ± EPM performed with five to six mice per group <i>n</i> = 5–6.