FGFC maintained the pluripotency of human ESCs in long-term culture.

<p>hESCs were first maintained in E8 Medium with either bFGF (A) or FGFC (B) and then prepared for the <i>in vitro</i> differentiation assay. Differentiation into three germ lineages (neural, muscle, and liver) was detected by immunocytochemistry. The result of a single experiment is shown. TUJ1: anti-Class IIIβ-Tubulin, SMA: anti-human smooth muscle actin, AFP: anti-α-fetoprotein. no 1° Ab: negative control cells stained with each secondary antibody alone. Scale bar: 100 μm.