Behavioral summary in group housed versus single housed (3 months of social isolation) <i>L3mbtl1</i> null mutant and wildtype mice.

<p>The figure summarizes the following tests in group and single housed mice, as indicated (<b>A</b>) General activity/locomotion. (<b>B</b>) Depression/ forced swim. (<b>C</b>) Anxiety/open field test. (N = 8 group housed <i>L3mbtl1-/-</i>, N = 10 group housed <i>L3mbtl1+/+</i>, N = 10 single housed <i>L3mbtl1-/-</i>, N = 10 single housed <i>L3mbtl1+/+</i>). (<b>D</b>) Cognition/radial arm maze (N = 8 group housed <i>L3mbtl1-/-</i>, N = 11 group housed <i>L3mbtl1+/+</i>, N = 11 single housed <i>L3mbtl1-/-</i>, N = 10 single housed <i>L3mbtl1+/+</i>). Behavioral assays were conducted in the order from the least to the most stressful, and thus (i) activity/locomotion, followed by (ii) radial arm maze, followed by (iii) anxiety/open field, and (iv) depression / forced swim as the final test. All data shown as mean ± S.E.M., * P <0.05; independent-samples t-test after two-way ANOVA: for (A) locomotion: F_(1,34) = 3.807, P = 0.05, Bonferroni post test P <0.05 for single housed <i>L3mbtl1-/-</i> vs. <i>L3mbtl1+/</i> +); for (B) forced swim: F_(1,34) = 10.93, P = 0.022, Bonferroni post test P <0.01 for single vs. group housed; for (C), (D) no significance. All data shown in Fig 2 are from batches of mice different from those shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121252#pone.0121252.g001" target="_blank">Fig 1</a>.