Expression of membrane-tethered GSK-3 increases embryonic levels of Arm.

<p>(A) Western blot comparing total Arm protein levels between five embryos expressing myr-GSK-3 and 5 wild-type embryos. (B) Western blot comparing total GSK-3 protein levels. Higher band represents expressed GSK-3 as this has a 3XHA tag, making it slightly larger than the endogenous GSK-3 directly below. (C) Cuticle of embryo expressing myr-GSK-3 kinase dead variant using ArmGal4. (D) Cuticle of embryo expressing myr-GSK-3 kinase dead variant using ArmGal4 in an embryo maternally and zygotically mutant for GSK-3.