Pulmonary IL-17⁺ γδ T cells are elevated IL-21R KO mice during IAV infection.

<p>WT C57BL/6 mice or IL-21R KO mice were infected with 0.1 LD₅₀ PR8 and lung cell suspensions were collected at various days post-infection as indicated. Cell suspensions were then stimulated with PMA/ionomycin for 4 hours in the presence of monensin, and IL-17 or IFNγ was measured by intracellular cytokine staining. (A) Cells were gated on Thy1.2⁺TCRδ⁺. Representative flow cytometry plots and quantification of frequencies and absolute numbers of cytokine positive γδ T cells are shown. (B-D) Quantification of frequencies and numbers of IL-17⁺ cells is shown. (B) total Thy1.2⁺TCRδ^-, (C) CD8⁺, (D) CD4+. (A-D) n = 4, combined from 2 independent experiments.