Dose-dependent effect of rHamOVGP1 on tyrosine phosphorylation of hamster sperm proteins.

<p>Hamster caudal epididymal sperm, cultured for 4 h in modified TALP medium alone or supplemented with different concentration of rHamOVGP1 (10, 20, 40, and 60 μg/ml), were processed for SDS-PAGE, transferred to PVDF membrane and probed with anti-phosphotyrosine antibody. A: representative blot; B: summary of data. Asterisks (*) indicate values that are significantly different (p<0.05) from that obtained with untreated sperm.