Methylene blue protects astrocytes against OGD-reoxygenation induced cell death.

<p>Quantitative analysis of astrocyte viability by Calcein AM assay <b>A.</b> Exposure of primary astrocytes to OGD-reoxygenation condition caused significant loss in viability and MB administration during OGD increased cell death. <b>B.</b> Administration of MB (0.1 μM and 1 μM) during reoxygenation significantly protected astrocytes from OGD-reoxygenation induced cell death. <b>C.</b> Representative images of Calcein AM staining at 24 hours after reoxygenation with or without MB treatment. <b>***</b> p < 0.0001 Vs. CTL. <b>#</b> p < 0.05, <b>##</b> and <b>###</b> p < 0.0001 Vs. OGD-reoxygenation control /0 μM MB.