mTORC1 activity is not required for antigenic recognition.

<p><b>a-b)</b> WT, T-Raptor^–/—and T-TSC2^–/—OT-II+ splenocytes were stimulated with OVA^323–339 for 24hrs. <b>a)</b> mTORC1 activity, as assessed by pS6^S240/244 expression was measured from the CD4+ population of each genotype. The bar graph below displays the MFI ± standard deviation (S.D.) of pS6 per genotype represented in above histogram plot. <b>b)</b> Expression of activation marker CD69, and homing marker, CD62L, are shown from the CD4+ population of each genotype. Below bar graphs indicate MFI of each molecule of interest from 3 independent experiments. <b>c)</b> 5c.c7 Rag^–/—CD4+ T cells were stimulated with PCC for 24hrs and expression of CD69 and CD62L was assessed from the 10% smallest and largest populations of activated CD4+ cells. Expression was compared against non-activated 5c.c7 Rag^–/—CD4+ T cells (CD4+ CD69–). Bar graphs below represent MFI ± S.D. of each molecule of interest from 3 independent experiments. The data are representative of (a,c) and/or are a composite of 3 independent experiments (b,c).