<p>A. Confocal fluorescence micrographs of worms fed the fluorescently labelled fatty acid derivative C<sub>1</sub>-BODIPY 500/510 C<sub>12</sub>. The panels at the top show parts of the intestines of adult worms; those at the bottom show first larval stage (L1) worms. The arrows point to the intestinal lumen where most of the dye is found in the mutants. Scale bars are 20 μ m. B. Graphs showing quantification (in arbitrary units) of the uptake of the dye into the cytoplasm of intestinal cells (Materials and Methods). Error bars indicate standard errors of the means. **** indicates significant difference in the means determined by one-way ANOVA and Dunnett's multiple comparisons test (σ = 0.05).</p
<p>The volumes of all BODIPY 493/503-positive droplets (fixative staining) in pharynx and tail regio...
<p>All images present <i>C. elegans</i> anterior to the left and show the pharynx and first part of ...
<p><b><i>A</i></b>, FRAP analysis of the AAT-6 dynamics was performed in 4-day old worms. Confocal i...
<p>A. Confocal fluorescence micrographs of worms fed the fluorescently labelled fatty acid derivativ...
<p>A. Micrographs of fixed young adult hermaphrodites stained with Oil Red O (top panels) or Sudan B...
<p>A) Worms were stained by culturing from the L1 stage to adult stage on a Nile Red-containing plat...
<div><p>(A−C) There is little change in fatty acid composition for <i>fat-5</i> (A), <i>fat-6</i> (B...
<p>, The localization of AAT-6<i><sup>1−517</sup></i>::GFP::AAT-6<i><sup>518–523</sup></i> was compa...
Example of a comparison of the same sample of worms taken under bright-field (A) and under 470 nm fl...
<p>The diameters of the two largest stained lipid droplets in the anterior intestine were measured f...
A) C. elegans dye uptake assay. In wild type worms, DiI dye is taken up by 6 pairs of amphid (head) ...
(a) Nile red fluorescence relative to length (TOF) in worms fed E. coli OP50 or MCB either alive or ...
Visualization of lipid droplets evidenced by BODIPY labeling in (A) wild-type young adults, (B) nhr-...
<p>(A) Fluorescence images of representative animals carrying the 1 Kb <i>ilys-3p</i>::<i>GFP</i> tr...
<p>(A–C) BODIPY 493/503 (fixative staining) fluorescence microscopy and corresponding bright-field m...
<p>The volumes of all BODIPY 493/503-positive droplets (fixative staining) in pharynx and tail regio...
<p>All images present <i>C. elegans</i> anterior to the left and show the pharynx and first part of ...
<p><b><i>A</i></b>, FRAP analysis of the AAT-6 dynamics was performed in 4-day old worms. Confocal i...
<p>A. Confocal fluorescence micrographs of worms fed the fluorescently labelled fatty acid derivativ...
<p>A. Micrographs of fixed young adult hermaphrodites stained with Oil Red O (top panels) or Sudan B...
<p>A) Worms were stained by culturing from the L1 stage to adult stage on a Nile Red-containing plat...
<div><p>(A−C) There is little change in fatty acid composition for <i>fat-5</i> (A), <i>fat-6</i> (B...
<p>, The localization of AAT-6<i><sup>1−517</sup></i>::GFP::AAT-6<i><sup>518–523</sup></i> was compa...
Example of a comparison of the same sample of worms taken under bright-field (A) and under 470 nm fl...
<p>The diameters of the two largest stained lipid droplets in the anterior intestine were measured f...
A) C. elegans dye uptake assay. In wild type worms, DiI dye is taken up by 6 pairs of amphid (head) ...
(a) Nile red fluorescence relative to length (TOF) in worms fed E. coli OP50 or MCB either alive or ...
Visualization of lipid droplets evidenced by BODIPY labeling in (A) wild-type young adults, (B) nhr-...
<p>(A) Fluorescence images of representative animals carrying the 1 Kb <i>ilys-3p</i>::<i>GFP</i> tr...
<p>(A–C) BODIPY 493/503 (fixative staining) fluorescence microscopy and corresponding bright-field m...
<p>The volumes of all BODIPY 493/503-positive droplets (fixative staining) in pharynx and tail regio...
<p>All images present <i>C. elegans</i> anterior to the left and show the pharynx and first part of ...
<p><b><i>A</i></b>, FRAP analysis of the AAT-6 dynamics was performed in 4-day old worms. Confocal i...